FIGURE 1.

Astilbin treatment significantly attenuated IMQ‐induced psoriasis‐like lesions in mice in a dose‐dependent manner. SKH‐1 mice were treated daily with IMQ or control cream on the back skin for 12 days. On Day 6, IMQ‐treated mice were randomized into five groups and topically apply calcipotriol ointment, ointment bases (OB), 0.2% astilbin ointment (AL) or 0.5% astilbin ointment (AH) were applied topically after IMQ application for 0.5 h. (A) Representative back skin from the six groups is shown. (B–E) Psoriasis Area Severity Index (PASI) score of back skin lesions in the four groups including erythema, scaling and infiltration is scored daily on a scale from 0 to 4, and the total score is calculated. Data are represented as the mean ± SEM. (n = 5). (F) Representative haematoxylin and eosin staining (×200) analyses of mouse back skin from the different treatment groups and epidermal thickness are measured using CaseViewer 2.1 software (scale bar, 100 μm). (G) The epidermal thickness of the different treatment groups was statistically analysed. Data are represented as the mean ± SEM (n = 5), and one‐way ANOVA tests were used for comparison of the significant differences (***p < 0.001)