Skip to main content
. 2022 Jan 12;26(4):1281–1292. doi: 10.1111/jcmm.17184

FIGURE 5.

FIGURE 5

Topical astilbin decreased IMQ‐induced mDCs in mice in a dose‐dependent manner. (A) Flow cytometry was performed to analyse the expression change of CD11+MHCⅡ+ DCs of mouse back skin in the different groups. (B) The rate of CD11+MHCⅡ+ DCs expression was statistically analysed. Data are expressed as the mean ± SEM (n = 5, **p < 0.01 and ***p < 0.001). (C) Western blot analysis of MyD88 expression in R837‐induced BMDCs pretreated or not with astilbin. Actin was used as a loading control. Protein amounts were normalized to the amount of actin and were quantified using the densitometry function of ImageJ software. The results are the mean ± SD of three independent experiments. **p < 0.01, ***p <0.001. (D) The levels of MyD88 in skin lesions of the different treatment groups were assessed by Western blotting. Actin was used as a loading control. Protein amounts were normalized to the amount of actin. The results are the mean ± SD of three independent experiments. (**p < 0.01, ***p < 0.001)