Fig. 3.

Immunofluorescence microscopy analysis of Vero cells expressing either the wild-type (WT) or the mutant BEND4 proteins. The weak staining in vector-only transfected cells (pCDNA4/TO) illustrates the level of endogenous BEND4 expression in Vero cells. The cellular distribution of the WT-BEND4 differed from the distribution of the mutant BEND4 protein. The WT-BEND4 protein formed a distinct rim around the nucleus with some network-like cytoplasmic distribution pattern while the mutant BEND4 had a significant loss in its cytoplasmic distribution and a more pronounced nuclear localization without a distinct rim.