Table 1.

The results of MALDI-TOF/TOF analysis for regulated protein spots

SSP No.	AC No.	Best protein Acc	Best protein score	Expect	Matches	Seq. cov., %	Best protein description	Upregulated in	Downregulated in
105	P62258	1433E_HUMAN	294	8.1e–026	21	56	14-3-3 protein epsilon	Wild-type and mutant
303	Q15293	RCN1_HUMAN	146	5.1e–011	9	18	Reticulocalbin-1	Mutant
502	P27797	CALR_HUMAN	743	1e–070	31	47	Calreticulin		Wild-type and mutant
3205	P07195	LDHB_HUMAN	133	1e–009	10	15	l-lactate dehydrogenase Β chain	Wild-type and mutant
5104	P30040	ERP29_HUMAN	338	3,20E–30	17	32	Endoplasmic reticulum resident protein 29	Mutant
5502	P50395	GDIB_HUMAN	146	5,10E–11	17	28	Rab GDP dissociation inhibitor beta		Wild-type

The TOF spectra were recorded in the positive ion reflector mode with a mass range from 400 to 2,000 Da. The Peptide Mass Fingerprints (PMFs) search was performed using MASCOT version 2.5 (Matrix Science). The following search criteria were used: species restricted to human; enzyme of trypsin; at least two independent peptides matched; at most one missed cleavage site; MS tolerance of ±50 ppm and MS/MS tolerance of ±0.4 Da; fixed modification being carbamidomethyl (Cys) and variable modification being oxidation (Met); peptide charge of 1+; and being monoisotopic. Only significant hits, as defined by the MASCOT probability analysis (p < 0.05), were accepted