Fig. 1.

Flow cytometric analyses. A Conversion of myeloid blasts into DC_leu through blastmodulatory Kit-I and Kit-M. Exemplary plots show DC_leu, characterised by the co-expression of the blast marker CD117 and the DC marker CD206, in uncultured WB, WB^DC(Control), WB^DC(M), and WB^DC(I). B Gain of maturation through Kit-I and -M. Exemplary plots show DC_mat, characterised by the co-expression of the DC marker CD206 and the maturation marker CCR7, in WB^DC(Control), WB^DC(M), and WB^DC(I). C Gating of IFNy-secreting T^CD3+ (CD3⁺IFNy⁺), T^CD4+ (CD3⁺CD4⁺IFNy⁺), and T^CD8+ (CD3⁺CD4⁻IFNy⁺) cells as detected by CSA. D Comparison of IFNy-secreting T^CD3+ (CD3⁺IFNy⁺) cells in WB^{DC(Control)-MLC}, WB^DC(M)-MLC, and WB^DC(I)-MLC. Abbreviations of all cell types are given in Table 2.