Table 3.

DC/DCleu-generating protocols

DC/DCleu protocol	DC/DCleu source	Composition (total)	Time of addition	Time of culture	Reference
Pici-PGE1	MNC	GM-CSF 500 U/mL	d0	9 days	[38]
		IL-4 250 U/mL	d0
		OK-432 10 μg/mL	d7
		PGE1 1 μg/mL	d7
Kit-I	WB	GM-CSF 800 U/mL	d0, d2–3	7–8 days	[Unpublished data]
		OK-432 10 μg/mL	d0, d2–3		European Patent No. 15 801 987.7-1118
Kit-M	WB	GM-CSF 800 U/mL	d0, d2–3	7–8 days	[Unpublished data]
		PGE1 1 μg/mL	d0, d2–3		European Patent No. 15 801 987.7-1118
Mode of action	GM-CSF	Induction of myeloid and DC differentiation			[16, 38]
	IL-4	Induction of DC differentiation
	OK-432	Danger signalling, stimulation of DC differentiation
	PGE1	Danger signalling, stimulation of DC maturation and migration (via CCR7 expression)

GM-CSF, granulocyte-macrophage colony-stimulating factor; IL-4, interleukin 4; OK-432, Picibanil; PGE₁, prostaglandin E₁; d day.