KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| T-PER Tissue Protein Extraction Reagent | Thermo Fisher Scientific | Cat# 78510 |
| protease inhibitor (1 pill/100ml T-PER) | Sigma-Aldrich | Cat# S8820 |
| DPP-4 inhibitor (20μl/ml T-PER) | Millipore-Sigma | Cat# SKUDPP4 |
| BCA protein analysis | Pierce-Fisher | Cat#23225 |
| FailSafe PCR PreMix | Lucigen Corp, Middleton,WI | Cat# FS99250 |
| SYBR Safe | Invitrogen | Cat# S33102 |
| TRIzol reagent | Thermo Fisher Scientific | Cat# 15596026 |
| PureLink RNA mini kit | Thermo Fisher Scientific | Cat# K157001 |
| RNeasy Mini kit | Qiagen | Cat#74106 |
| iScript cDNA synthesis kit | BioRad | Cat# 1708891 |
| SsoAdvanced Universal Probes Supermix | BioRad | Cat# 1725281 |
| Critical commercial assays | ||
| Dual Dual-Luciferase Assay System | Promega | Cat# E1910 |
| Ultra-Sensitive Mouse Insulin ELISA | Crystal Chem | Cat# 90080 |
| QuantiChrom Iron Assay | BioAssay Systems | Cat# DIFE-250 |
| Hepcidin-Murine CompeteTM ELISA | Intrinsic Life Sciences | Cat# HMC-001 |
| V-PLEX GLP-1 Total Kit | Meso Scale Discovery | Cat# K1503PD |
| TaqMan gene expression Assay - Hif2a (Epas1) | Thermofisher Scientific | Mm01236112_m1 |
| TaqMan gene expression Assay - Hif1a | Thermofisher Scientific | Mm00468869_m1 |
| TaqMan gene expression Assay - Vhl | Thermofisher Scientific | Mm00494137_m1 |
| TaqMan gene expression Assay - Dmt1 (Slc11a2) | Thermofisher Scientific | Mm00435363_m1 |
| TaqMan gene expression Assay - Dcytb (Cybrd1) | Thermofisher Scientific | Mm01335930_m1 |
| TaqMan gene expression Assay - Phd2 (Egln1) | Thermofisher Scientific | Mm00459770_m1 |
| TaqMan gene expression Assay - Neu3 | Thermofisher Scientific | Mm00479379_m1 |
| TaqMan gene expression Assay - Pdk1 | Thermofisher Scientific | Mm01276567_m1 |
| TaqMan gene expression Assay - Pgk1 | Thermofisher Scientific | Mm00435617_m1 |
| TaqMan gene expression Assay - Glut1 (Slc2a1) | Thermofisher Scientific | Mm05908127_s1 |
| TaqMan gene expression Assay - Rpl32 | Thermofisher Scientific | Mm07306626_gH |
| TaqMan gene expression Assay - Actb | Thermofisher Scientific | Mm02619580_g1 |
| Deposited data | ||
| Array data submitted to GEO database | This paper | GSE169403 |
| Experimental models: Organisms/strains | ||
| VhlF/F;Vilcre | Prof.dr. Y. Shah, Dept. Mol & Int Phys, University of Michigan | N/A |
| Hif2αF/F;VilWt/Wt, Hif2αF/F-VilCreERT | Prof.dr. Y. Shah, Dept. Mol & Int Phys, University of Michigan | N/A |
| HampF/F;AlbWt/Wt HampF/F-AlbCre | Prof.dr. Y. Shah, Dept. Mol & Int Phys, University of Michigan | N/A |
| Gcgstopflox | Prof.dr. R.J. Seeley, Dept. Surgery, University of Michigan | N/A |
| Glp1rstopflox | Prof.dr. R.J. Seeley, Dept. Surgery, University of Michigan | N/A |
| FVB.129S6Gt(ROSA) 26Sortm2(HIF1A/luc)Kael)/J – ODD-Luc | Jackson laboratory | Stock no.006206 |
| C57BL/6J | Jackson Laboratory | Stock no.000664 |
| Long-Evans rats | Harlan Laboratories | HsdBlu: LE |
| Oligonucleotides | ||
| Oligonucleotides are listed in Table S7 | N/A | N/A |
| Software and algorithms | ||
| Graphpad Prism | Graphpad Software | Version 8.1.2 |
| R A Language and Environment for Statistical Computing | R | R Core Team (2017) |
| Expression console v.1.3.0.187 | Thermo Fisher Scientific | N/A |
| CARMAweb | Medical University Innsbruck | Rainer et al. (2006) |
| QIAGEN Ingenuity Pathway Analysis, IPA | QIAGEN | www.qiagen.com/ingenuity |
| Other | ||
| Regular chow diet | Envigo Teklad | Cat# 7012 |
| 60% HFD | Research Diets, Inc | Cat# D12492 |
| Custom high fat (45% FDC) AIN-93G with 35ppm Fe | Dyets Inc, Pa | Cat# 115244 |
| Custom high fat (45% FDC) AIN-93G with 350ppm Fe | Dyets Inc, Pa | Cat# 115245 |
| Osmolite OneCal liquid diet | Abbott, Columbus, OH | Cat# 64633 |
| Ensure Plus | Abbott | Cat # 57263 |
| Accu-Chek Aviva Meter | Accu-Chek, Roche Diabetes Care | https://www.accu-chek.com |
| Endopath ETS-FLEX 35mm Stapler | Ethicon endo-surgery | VASECR35 |
| Nuclear Magnetic Resonance | EchoMRI LLC, USA | EchoMRI-900 |
| Ovation PicoSL WTA System V2 | Nugen | Part no. 3312 |
| GeneChip Rat Gene 2.1 ST Array Plate | Thermofisher Scientific | Cat# 902143 |