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. 2021 Dec 9;43(1):28–39. doi: 10.1093/carcin/bgab118

Figure 3.

Figure 3.

Attenuation of HSD11B2 promotes tumor growth and invasion. (A) Confirmation of HSD11B2 knockdown in siRNA-treated CAL27 and CA83 cells by RT–qPCR. (B) Quantification of cortisol in siRNA-treated CAL27 and CA83 cells by ELISA. (C) Gene expression analyses of Egfr, VegfA in scrambled control or siRNA-treated CAL27 cells. (D) Percentage of proliferation of the siRNA-treated CAL27 and CA83 cells as assessed by Alamar Blue assay. (E) Representative images of in vitro scratch assay upon treatment of CAL27 and CA83 cells with siRNA showing time dependent gap closure. (F) Plot of percent migration. For all experiments, error bars represent standard error of the mean (SEM) from n = 5 biological samples per group each from two independent experiments; *P < 0.05, **P < 0.01 and ***P < 0.005 for comparisons between control and siRNA-treated cells using Student’s t-test.