Figure 5.

Serum IgG (A), IgG1 (B), and IgG2a (C) anti-rHagB response in mice immunized by the s.c. route with rHagB alone or with GPI-0100 or a saponin adjuvant. Mice were immunized on days 0, 14 and 28. Serum samples were collected prior to each immunization and at 6 weeks after the initial immunization. Values are expressed as mean ± SEM. Statistical significance in IgG, IgG1, and IgG2a antibody responses was evaluated by t tests (with unpaired, nonparametric and Mann-Whiteny test). * P < 0.05, and ** P < 0.01 compared with mice immunized with rHagB alone, # P < 0.05, compared between the indicated groups.