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. 2022 Feb 10;139(6):876–888. doi: 10.1182/blood.2021013832

Figure 1.

Figure 1.

BM stromal cells of precursor stages of MM disease are more prone to differentiate into adipocytes and increase the proliferation of myeloma cells. (A) Representative fluorescence-activated cell-sorting plots of BMSCs from HD and patients with MGUS/SMM and NDMM showing increased expression of adipogenic lineage markers at baseline. (B) Quantification of panel A. (C) Representative Oil Red O staining (C) and associated quantification of neutral lipids (D) from BMSC-derived adipocytes from HD and patients with MGUS/SMM and NDMM cocultured in the presence or absence of MM.1S cells in direct contact or in transwells. (E) Proliferation of MM.1S cells cocultured in the presence of pre-adipogenic BMSCs from HD and patients with MGUS/SMM and NDMM. (F) Proliferation of MM.1S cells cocultured with mature adipocytes differentiated from BMSCs from HD and patients with MGUS/SMM and NDMM. (G) Relative proliferation of 5TGM1 cells after 72 hours of coculture with MC3T3-E1 pre-osteoblasts or OP9 pre-adipocytes. Biospecimens from MGUS and SMM from MGUS and SMM patients were analyzed independently but were pooled together for analysis purposes. All data indicate n ≥ 3; mean ± standard error of the mean. *P < .05.