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. 2022 Jan 28;12:826112. doi: 10.3389/fphar.2021.826112

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Copyright © 2022 Speck, Kleinau, Meininghaus, Erbe, Einfeldt, Szczepek, Scheerer and Pütter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Large-scale purification of RXFP1 constructs. (A) SEC chromatogram of pVL-11 2L large-scale purification. (B) SDS-PAGE shows the course of pVL-11 purification, the expected size of the cut construct is 76 kDa (M = size standard, L = lysate, S = solubilizate, TF = flow through, E = M2 eluate, C= concentrated M2 eluate as SEC input, V = void volume peak, A = aggregate peak fraction, I = pool fraction I monomeric peak RXFP1, II = pool fraction II monomeric peak RXFP1). (C) nanoDSF melting curve of pVL-11 aggregate peak fraction. (D) nanoDSF melting curve of monomeric GPCR from the pool fraction I.