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SPR sensograms of relaxin binding to different RXFP1 constructs. (A) Dose-dependent binding curves of relaxin (by adding with 3.125, 6.25, 12.5, 25, 50, and 100 nM relaxin concentration to the receptor fixed on the SPR chip) for RXFP1 pVL-11(FLAG-eGFP-M82-S707- AVI), and (B) for RXFP1 pVL-13(FLAG-eGFP-P391-S707-AVI). The red lines represent kinetic fits. Experiments were performed in triplicates.
