FIG. 1.

Southern hybridizations of EcoRI-digested genomic DNAs of strains from eight onychomycosis patients (P1 to P8) with T. rubrum infection. A PCR-amplified fragment (∼1,220 bp) of 18S rDNA plus the adjacent ITS region from a T. rubrum strain was used as a probe. The numbers above the lanes represent the RFLP types found among T. rubrum strains. Five distinct RFLP types (1 to 5) are shown. Lanes marked by solid lines represent strains obtained from the same patient during the course of therapy. Example lanes are not in chronological order. Types 2 and 4 have double bands in the 4.3- to 6.5-kb region. The leftmost lane contains a molecular weight marker (HindIII-digested lambda DNA).