Table 2.
Primer sequences of housekeeping and target genes concerned with intestinal barrier function
| Item1 | Sequences (5′ to 3′)2 | Length, bp | Tm, °C |
|---|---|---|---|
| Occludin |
F: GTGGGACAAGGAACGTATT R: TCTCTCCGCATAGTCCGAA |
115 | 60 |
| Claudin-1 |
F: ATACAGGAGGGAAGCCAT R: ATATATTTAAGGACCGCCCTCT |
89 | 60 |
| ZO-1 |
F: GCTCAGCCCTATCCATCT R: GGACGGGACCTGCTCATAA |
90 | 60 |
| Mucin-1 |
F: GTGCCGCTGCCCACAACCTG R: AGCCGGGTACCCCAGACCCA |
141 | 60 |
| Mucin-2 |
F: CAGACCTACTCAGAGTTCCT R: CTCGGGCTTGTTGATCTT |
84 | 60 |
| GAPDH | F: CAGCAATGCCTCCTGTACCA | 72 | 60 |
| R: ACGATGCCGAAGTTGTCATG |
1ZO-1, zonula occludens-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase
2F, forward primer; R, Reverse primer