FIG. 3.

Epitope mapping of MAb 3-3D by Western blotting. (A) Bacterial lysates containing GST-fusion peptides corresponding to aa positions 631 to 671, 672, 673, or 674 of NP (subtype Zaire) were examined for their reactivities to MAb 3-3D. (B) Bacterial lysates containing GST-fusion peptides corresponding to aa positions 648, 649, 650, or 651 to 673 of NP (subtype Zaire) were examined for their reactivities to MAb 3-3D. To show the similar levels of expression of each fusion peptide, a duplicate membrane was stained with anti-GST antibody.