Fig. 3.

Cytokines and platelet microparticles in plasma of COVID-19 patients. (A) IL-6 and (B) TNFα were measured in plasma of above patients using CBA Array. Gating strategy is described in Fig. S3. Each dot represents individual value. The red dots (in case of ICU patients) and dark green dots (asymptomatic) represents the individuals (n = 9 each) used for platelet transcriptome analysis. Data are the mean ± SEM. One-way ANOVA was used to compare between the groups, *P < 0.05, ** < 0.01, ****P < 0.0001 and ns = non-significant. (C) Western blotting of platelet lysates for phospho/non-phospho p38 and ERK from 4 representative images in each asymptomatic and ICU patient. Densitometry data of MAPKs were normalized with β-actin and presented as fold change of phospho/non-phospho (D) p38 and (E) ERK from asymptomatic (n = 6), ICU patients (n = 9 and 8 for p38 and ERK respectively). Unpaired t-test was used to compare between the groups, *P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)