Fig. 6.

Rab5b knockdown in MLE-15 cells decreases mature SP-B production. MEL-15 cells were transduced with lentiviruses that express nontargeted control shRNA or Rab5b-specific shRNA sequences (shRNA1, shRNA2) after selection for 5 d in puromycin. (A) Western blot analysis of transduced MLE-15 cells using antibodies to detect RAB5B, pan-RAB5 (total RAB5), mature SP-B, proSP-B, and ACTIN. (B) Quantification of the western blot signal for RAB5B, total RAB5, mature SP-B, and proSP-B from MLE-15 cells transduced with control shRNA, Rab5b shRNA1, and Rab5b shRNA2 from four independent transductions. Multiple medians were compared using the Kruskal–Wallis test followed by Dunn’s multiple comparison. ns, not significant. ***P < 0.001.