Fig. 6.

Epigenetic state controls additional TLRs and correlates with basal inflammatory state and basal immune infiltration. (A) Change in expression of TNFAIP3 as measured by qPCR after treatment with the indicated agonists for 6 h in BE2(c) cells after treatment with vehicle or dox for 14 d. (B) Comparison of the MES or ADRN signature scores (35) to the enrichment of the hallmark inflammatory response signature and to a cytotoxic T cell infiltration signature (82) in 498 neuroblastoma tumors (83). Data were obtained from and analyzed in R2 (https://hgserver1.amc.nl:443/). (C) Comparison of the MES or ADRN signature scores to the enrichment of the hallmark inflammatory response signature in 23 neuroblastoma cell lines (data from GSE28019). Data were obtained from and analyzed in R2 (https://hgserver1.amc.nl:443/). (D) Relative enrichment of the hallmark inflammatory response signature in the indicated neuroblastoma cell lines as measured by QuantSeq. BE2(c) cells expressing inducible Luc control or PRRX1 were treated with vehicle or dox for 14 d. (E) Relative infiltration of xenograft tumors from the indicated cell lines with the indicated immune cell types, as measured by flow cytometry and presented as percent of live cells. DC, dendritic cell. An ANOVA comparing biological replicates was performed; *P < 0.05, **P < 0.02, and ***P < 0.001. Error bars represent SEM between biological replicates. NS, not significant.