Fig. 5.

Knockdown of liver Cul3, but not Cul1, increases IRS abundance and glucose tolerance in vivo. Chow-fed male C57BL/6J mice at 9 wk of age were intraperitoneally injected with AAV8-Null, AAV8-shCul1, or AAV8-shCul3 (2 × 10¹¹GC/mouse). GTT was performed at 4 to 5 wk postinjection. (A and B) Liver protein levels. Relative IRS1 protein densitometry is shown in the Right panel. (C–F) GTT and glucose area under the curve of GTT. All results are expressed as mean ± SEM (n = 4 to 5). *P < 0.05. Unpaired Student’s t test was used to calculate the P value.