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Knockout of Nrf2 abolished the regulatory capability of Daph for ovarian senescence-associated protein expression. (A–D) The IHC staining showed the p16 and klotho expressions in the five groups in Nrf2−/− mice. (E,F) Expressions of p16 and klotho were measured through western blot. The data in (A–D) are shown as means ± SEMs, n = 5. The data in (E,F) are shown as means ± SEMs, n = 3. Statistical significance: **p < 0.01.
