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. 2022 Feb 4;119(6):e2111380119. doi: 10.1073/pnas.2111380119

Fig. 3.

Fig. 3.

Oncogene iASPP and TMCO1 are associated with each other in vivo in colon cancer tissues. (A and B) Representative WB of Gp78 and iASPP proteins in the same set of colon cancer tissues described in Fig. 1B were presented. α-tubulin was used as a loading control (same as the blots shown in Fig. 1B). (C) The scatter diagram showed the lineal correlation of TMCO1/Gp78 (P = 0.473, C) in colon cancer tissues. (D) The key proteins that bind with Gp78 was identified by mass spectrum assay following Gp-78 pull-down. After that, the top three fragments of identified peptides derived from the target proteins (Gp78, CP3A4, TMCO1, and iASPP) were used for quantification. (E) Quantification of iASPP WB bands in tumor (T) and the paired normal control (N) were shown in the plot. (F) The correlation between iASPP protein levels and tumor stages was shown in the box plot. (G) The scatter diagram showed the lineal correlation of TMCO1/iASPP (P = 0.014) in colon cancer tissues. N.S., not significant; *P < 0.05; **P < 0.01 (B, E, and F).