Fig. 6.

Ca²⁺-channel protein TMCO1 is required for the regulation of ER Ca²⁺ content by iASPP. (A–F) Representative WB images showing the efficiency of iASPP OE (A and B), KD (C and D), iASPP OE, or/and TMCO1 KD (E and F) in HT-29 and HCT-116 cells. β-actin was used as a loading control. ER Ca²⁺ stores in HT-29 (n = 20) and HCT-116 (n = 20) cells were measured by loading with 5 μM Fura-2 AM, following ionomycin (1 μM) in Ca²⁺-free medium. The quantification of AUC derived from at least three independent experiments are presented as mean ± SEM in the bar graph at Bottom. Values in control cells were normalized to 1. *P < 0.05; **P < 0.01; N.S., not significant (A–F).