Figure 1. Behavioral data for young mice on experimental diet.

(A) Experimental timeline. (B) The diet did not alter weight gain in any group, but males weighed more than females and YAC128 mice weighed more than WT. (C) There was no anxiety phenotype detected in any group based on time spent in the open zone of the EZM. (D) In WT females, the high Mn diet induced hyperactivity measured by distance travelled during the EZM. (E) WT females on high Mn diet were hyperactive as measured by total distance travelled over 30 min in locomotor activity chambers (post-hoc Sidak’s multiple comparisons). (F) There were no impairments in rotarod performance and motor learning was intact indicated by significant improvement over training days. Days that do not share a superscript are significantly different from each other. (G) Grip strength was similar among all genotype and diet groups. For all, mean ± S.E.M. plotted unless otherwise noted. n=5-7 per genotype-diet group for females and n=5-11 for males. *P<0.05 indicates main effect of genotype. #P<0.05, ##P<0.01 indicates significant effect of Mn within genotype following a significant interaction (post-hoc Sidak’s multiple comparisons).