Skip to main content
. 2022 Jan 31;119(6):e2117554119. doi: 10.1073/pnas.2117554119

Fig. 3.

Fig. 3.

Induced degradation of Myo3 and Myo5 inhibits the colocalization of Atg11 and Atg40. (A) Fluorescence images of yeast cells expressing Myo3-AID1, Myo5-AID2, Atg40-3xGFP, and Atg11-2xmCherry before and after 3-h rapamycin (Rapa.) treatment. Cells were pretreated with either EtOH or IAA for 90 min before the addition of rapamycin. The arrowheads point to Atg11 puncta that colocalize with Atg40 puncta. (Scale bars, 2 μm.) (B) The percentage of Atg11 puncta that colocalize with Atg40 was quantified. Error bars represent SD, n = 3 independent experiments. ns, nonsignificant (P ≥ 0.05); *P < 0.05. Student’s t test.