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Mutational analysis of the U1-SL4 RNA. (A) EMSA experiments performed with SF3A1-UBL and U1-SL4. (B) Loop mutant of U1-SL4 probed for binding to SF3A1-UBL. Bases different from WT U1-SL4 are shown in red. (C) Binding curves of the indicated U1-SL4 variants. (D–F) Mutants of the upper helical part of U1-SL4. Respective Kd values were derived from curve fitting to the relative bound fraction per lane. GST, glutathione-S-transferase.
