Table 2.
Affinities and splicing activity of SF3A1-UBL mutants measured by SPR spectroscopy and in vivo reporter assays, respectively
| SF3A1-UBL construct | Kd [μM] | Fold increase of Kd compared to WT | Average exon 2 inclusion (%) | ΔPSI |
| WT | 0.332 ± 0.03 | 1.0 | 57.46 | 0.00 |
| K717A | 1.586 ± 0.27 | 4.9 | 50.05 | 7.41 |
| K754A | 16.733 ± 4.38 | 52.0 | 46.78 | 10.68 |
| F763A | 2.764 ± 0.3 | 8.6 | 49.25 | 8.21 |
| K765A | 7.889 ± 0.71 | 24.5 | 50.19 | 7.27 |
| K786A | >50 | >167 | 44.73 | 12.73 |
| R788A | >25 | >83 | 42.97 | 14.49 |
| R788K | >25 | >83 | n.d. | n.d. |
| RGG2AAA | >50 | >167 | 50.52 | 6.94 |
| G789I | 41.9 ± 5.7 | 130.1 | 46.12 | 11.34 |
| G790I | 14.1 ± 3.12 | 43.8 | 49.65 | 7.81 |
| R791A | 5.97 ± 0.5 | 18.5 | 48.98 | 8.48 |
| KK2AA | >25 | >83 | 51.76 | 6.38 |
| RKK2AAA | n.d. | n.d. | 51.76 | 5.7 |
| K754A/RGG2AAA | n.d. | n.d. | 42.33 | 15.13 |
| K765/RGG2AAA | n.d. | n.d. | 42.18 | 15.28 |
See SI Appendix, Fig. S3 for the sensograms of the SPR measurements. See Fig. 5 for primer extension analysis that yield the exon 2 inclusions. n.d., not determined (55).