Fig. 2.

Localization of PAR₂-muGFP in the intestine and DRG by immunofluorescence and RNAScope in situ hybridization. (A and B) Localization of GFP immunoreactivity in duodenum and jejunum (A) and ileum and colon (B) of Par₂-mugfp and wild-type mice. (C) RNAScope localization of F2rl1 and Gfp mRNA in colon of Par₂-mugfp and wild-type mice. Arrowheads indicate immunoreactivity at the basolateral membrane of epithelial cells and mRNA expression within epithelial cells. (D and E) RNAScope localization of F2rl1 and Gfp mRNA in DRG of Par₂-mugfp (D) and wild-type (E) mice. (Scale bars, 10 µm.) Representative images, independent experiments. n = 5 mice.