Skip to main content
. 2022 Feb 7;119(6):e2115379119. doi: 10.1073/pnas.2115379119

Fig. 5.

Fig. 5.

Stabilization of pH switch regions prevents low-pH–triggered cell–cell fusion. HA fusogenicity as measured in a cell–cell fusion assay in HEK293 cells by cotransfection of plasmids encoding WT or stabilized HA (H1-SC18, H1-MI05, H3-WI05, and H3-HK68), human TMPRSS2, and GFP. Overlays of GFP and brightfield channels 24 h after transfection are shown before (pH 7.4) and 1 h after (pH 5.0) exposure to low-pH medium to trigger fusion.