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. 2022 Feb 4;119(6):e2117300119. doi: 10.1073/pnas.2117300119

Fig. 3.

Fig. 3.

A dorsoventral gradient in spatial representation. (A) Reconstruction of recording sites. (Left) DiI-labeled probe track in the prefrontal cortex (arrow). (Right) Location of silicon probes. Recording site for each mouse is presented in a different color (n = 20 mice). M2: accessory motor cortex, PL: prelimbic cortex, CG: cingulate cortex, ORBm: medial orbital cortex, a: anterior, p: posterior, d: dorsal, v: ventral. (B) Example of the dorsoventral depth profile of theta power along the shank. (C) Average normalized theta power as a function of d–v depth (R = −0.995, P = 10−63). (Inset) Average normalized theta power of the dorsal versus the ventral recording site (P = 0.0004, Wilcoxon signed-rank test, n = 16 mice). (D, Left) Identification of unit location along the probe shank. Gray and green depths indicate the locations used for grouping neurons into dorsal and ventral mPFC, respectively. (D, Right) Comparisons of the mean SI (P = 0.03) and the proportion of neurons with a significant SI for dorsal and ventral groups of cells recorded in individual mice (paired t test with Bonferroni correction for three comparisons, n = 17 mice). (E) The same comparisons as in D during exploration of the nov track (P = 0.006, n = 11 mice, paired t test). (F) Comparable SI across cortical layers. (Top) Histological examples of recording sites in superficial layers (2/3, Left) and deep layers (5/6, Right). (Right) There was no correlation of the mean SI (P = 0.256) or proportion of neurons with a significant SI (P = 0.422) and laminar depth of the recording in individual mice (Spearman’s r, n = 20 mice). (G) Comparable mean SIs for superficial and deep-layer recordings (P = 0.076, Mann–Whitney U test, n = 6 and 17 mice). (H) The mean SI obtained from recordings in individual mice did not depend on the recording location in the left or right hemisphere (P = 0.161, n = 13 and 10 mice for left and right, respectively; unpaired t test). Points in C–H represent average values for individual mice. Circles combined with lines represent data from individual mice. Continuous lines in C represent means across animals ± SEM.