Fig. 4.

Salt diminishes the difference in FE between pro-IL-1β and mature IL-1β. (A) Energy landscape of pro-IL-1β and mature IL-1β as they travel through the GSDMD pore at two salt concentrations, 0.05 M and 0.15 M. (B) Relative release rate. (Definition is the same as Fig. 3.) Error bars in A and B were obtained by averaging over 10 random cargo orientations. (C) Calculation of the characteristic shielding length of the GSDMD pore. FE values for pro-IL-1β at 0.15 M were used. With the logarithmic of the FE as the vertical axis and z as the horizontal axis, the slope is −1/L. (D) Immunoblots of pro-IL-1β and mature IL-1β released through GSDMD pores from liposomes at two salt environments (0.05 M and 0.15 M) over 2-h time windows.