Figure 1.

DOX-induced cytotoxicity was inhibited by CME in H9C2 cells and primary cultured cardiomyocytes. (A) H9C2 cells were pretreated with 0.3 or 1 mg/mL CME. After 2 h, the cells were treated with 1 µM DOX for 24 h. Cell viability was investigated by MTT assay. (B,C) H9C2 cells and primary cultured cardiomyocytes were pretreated with 1 mg/mL CME for 2 h and then treated with 1 µM DOX for 24 h. (D–F) MDA-MB-231 cells (D), H1299 cells (E), and HT29 cells (F) were pretreated with 1 mg/mL CME. These cells were stimulated with 1 µM DOX for 48 h. Cell viability was measured by MTT assay. Values are presented as the mean ± SEM of three individual experiments.