Figure 2.

Breast tumors of obese mice display higher lac/pyr ratio than tumors of lean mice. (A) Representative MRS spectrum after injection of hyperpolarized 1-¹³C pyruvate acquired in vivo. (B) Representative evolution of ¹³C pyruvate and ¹³C lactate peaks over the time, from which the area under the curve and ratios are calculated. (C) Tumor size at the time of HP 1-¹³Cpyruvate-to-lactate spectra acquisition of E0771 tumors measured by T2-weighted MRI images. (D) Calculated Lac/Pyr ratio in fasted mice and (E) mice challenged with glucose. Sample size: n = 7 for ND and n = 7 for HFD, failure to acquire spectrum from a mouse from HFD. Statistical analysis: unpaired t-test; ns p > 0.05; ** p < 0.01.