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. 2022 Jan 23;14(3):562. doi: 10.3390/cancers14030562

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Breast tumors of obese mice produce more lactate than tumors of lean mice. (A–E): E0771 cell line; sample size: n = 6 for ND and n = 8 for HFD. (F–J): PY8119 cell line; sample size: n = 7 for ND and n = 5 for HFD. (A,F) Tumor size at the time of the necropsy. (B,G) ¹³C lactate and (C,H) ¹³C alanine concentrations from tumor extracts. (D,I) Correlation curve between the bodyweight of the mice and tumor ¹³C lactate (E) and ¹³C alanine (F,J) production. Statistical analysis: unpaired t-test; *** p < 0.001.