Figure 4.

Spectral imaging with linear unmixing allows for real-time identification of optically barcoded subpopulations. (A) Schematic of the SILU analysis pipeline used to image optical barcodes in live cells. (B) Subpopulations with barcodes indicated at left were sorted, and their fluorescence signatures were corroborated via SILU. (C) CPP35-L6 cells were stained for E-cadherin to demonstrate the compatibility of far-red fluorochromes with the OBC panel, enabling seven-colour applications. In merged panel, OBC channels have been converted to greyscale. SILU, spectral imaging with linear unmixing.