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The capability of cultured glioma (SW1088), glioblastoma (A172), and neuroblastoma (SH-SY5Y) cells to withdraw leucine from culture media (minimal medium supplemented with 25 mM glucose and 0.8 mM leucine-13C6) and to release KIC. LC-MS analysis of the culture media allowed estimation of specific uptake of Leu (a), the time dependence of KIC release (b), the specific release of KIC (c), and calculation of the ratio of released KIC to withdrawn Leu (d).
