Figure 3.

Levels of 3-hydroxybutyrate (3-OHB) in culture media from glioma (SW1088), glioblastoma (A172), and neuroblastoma (SH-SY5Y) cells were enzymatically estimated, and subsequently, the obtained values were used to calculate the specific release of 3-OHB (a). The effect of an increased level of leucine in culture medium (up to 8.8 mM) of neuroblastoma (SH-SY5Y) cells on their capacity to release 3-OHB (b). LC-MS was used to estimate the abundance of citrate isotopes released into the culture media from glioma cells (SW1088), which were incubated in a minimal medium supplemented with 25 mM glucose and either 0.8 mM leucine (12C-Leu) or 0.8 mM leucine-¹³C₆ (13C-Leu; (c)). Group M represents the normal molar mass of citrate (191,124 g/mol), and group M + 2 represents the molar mass of citrate increased by two units (193,124 g/mol).