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. 2022 Jan 24;14(3):585. doi: 10.3390/cancers14030585

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence photomicrographs of human glioma (SW1088), glioblastoma (A172), and neuroblastoma (SH-SY5Y) cells, which were double-labeled with antibodies against the α subunit of 3-methylcrotonyl-CoA carboxylase (MCC; green) and pyruvate dehydrogenase (PDH; red). Cell nuclei were visualized by DAPI. The merged views are represented separately with higher magnifications (merge). Negative control was performed by omitting the primary antibodies (negative control). The scale bars represent either 20 or 10 μm.