TABLE 2.

Comparison of rLipL32 IgG ELISA with standard diagnostic tests for leptospirosis

Days after initiation of symptomsa	No. of samples	Standard diagnostic tests				rLipL32 IgG ELISA
		Maximum reciprocal MAT titer, median (range)	No. (%) of samples		Whole-Leptospira IgM ELISA positiveb	OD450 median (range)	No. (%) giving positive reactionsc
			Reciprocal MAT, titer ≥ 100	Culture isolation
Acute-phase samples (n = 50)d
2–4	5	0 (0–200)	1 (20)	1 (100)e	1 (20)	0.008 (0.003–0.039)	0 (0)
5–7	23	0 (0–800)	7 (29)	6 (67)e	9 (41)	0.045 (0.005–1.181)	10 (43)
8–23	21	200 (0–3,200)	12 (60)	0 (0)e	16 (80)	0.176 (0.023–1.054)	18 (86)
Convalescent-phase samples (n = 50)
17–28	23	1,600 (200–6,400)	23 (100)	NA	21 (100)	0.501 (0.052–0.998)	23 (100)
29–35	19	1,600 (400–6,400)	19 (100)	NA	18 (95)	0.591 (0.008–1.050)	16 (84)
36–113	8	800 (200–800)	8 (100)	NA	8 (100)	0.344 (0.100–1.256)	8 (100)

^aDays between reported onset of illness and serum collection; one was missing. NA, not applicable. 

^bResults of the whole-Leptospira IgM ELISA were not available for two individuals' paired serum samples. Percentages shown are for the number of samples tested. 

^cPositive reactions were defined as absorbance greater than the 96th percentile among 50 serum samples from healthy individuals. 

^dAcute-phase serum samples were collected upon hospital admission. 

^eA total of 14 blood cultures were performed for 50 serum samples collected during the acute phase of illness; 1, 9, and 5 were performed for samples collected in the first 4, 5 to 7, and >7 days of illness, respectively.