TFF1 expression analyses in aqueous humor samples of RB patients and corresponding primary cell culture supernatants. (a) TFF1 ELISA analysis of 15 aqueous humor samples of RB patients, showing four patients with highly expressed TFF1 (>400 pg/mL), five patients with expressed TFF1 (30–400 pg/mL), and six patients without TFF1 expression (0–30 pg/mL) in the aqueous humor. (b) The supernatants of corresponding primary cell cultures of RB patient tumors shown in (a) revealed six highly TFF1 expressing samples, three samples expressing TFF1, and six samples without TFF1 expression. The supernatant of two RB cell lines (Rbl-13 and Rbl-30) highly expressing and secreting TFF1 are used as internal positive controls. Vertical dotted lines indicate three TFF1 expression levels; #: sample was a vitreous body aspirate.