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. 2022 Jan 28;14(3):677. doi: 10.3390/cancers14030677

Figure 2.

Figure 2

Western blot analysis of TFF1 expression in cell culture supernatants of primary cell cultures and aqueous humor samples of RB patients. (a) Western blot analysis of primary cell culture supernatants showing TFF1 expression in the highly TFF1 expressing tumors of the ELISA analysis (T06, T09, T14, and T18). Recombinant TFF1 (rTTF1) and the RB cells lines Rbl13 and Rbl30 served as positive controls. (b) TFF1 expression levels revealed by Western blot analysis for the four AH samples with high TFF1 expression levels in the ELISA assay. The indicated intensity ratios relative to ß-actin, used as a loading control, were calculated using MICRO MANAGER 1.4 software (University of California, San Francisco, CA, USA). -: empty lanes; na: not analyzed.