Figure 2.

Growth patterns and viability of TRAMP tumor organoid lines. Brigthfield microscope pictures of basal cell-derived (A) and luminal-cell-derived (B) organoid cultures treated with DMSO (upper panel) or enzalutamide (lower panel). Scale bars represent 500 µm. (C,D) Both luminal and basal cell-derived organoids exhibit significantly smaller organoid size upon treatment with 10 µM enzalutamide when compared to DMSO, although decreases in organoid size are more pronounced in luminal cell-derived organoids (left panels). Interestingly, luminal cell-derived organoids but not basal cell-derived organoids exhibit lower viability as determined by luminescence upon treatment with enzalutamide. Measurements as described in the methods section of five biological replicates with three technical replicates each are shown. Error bars represent SEM ((C,D) bar graphs).