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. 2022 Jan 19;12(3):231. doi: 10.3390/ani12030231

Table 4.

Effects of a precision biotic (Glycan PB) ingredient 1 supplemented at different doses in feed without or with the inclusion of a xylanase enzyme on the litter characteristics, foot pad lesions, and gait scores of broiler chickens raised in floor pens, evaluated at 35 d of age (Trial 1).

Xylanase Glycan PB Dose Litter Characteristics Welfare Assessment
Litter pH Total N
(mg/g)
Inorganic N
(mg/g)
Ammonia
(mg/g)
Ammonia/Inorg. N (mg/mg) Ammonia/Tot. N (mg/mg) Litter Score (1–5) Foot Pad Score 0 (%) Foot Pad Score 1–4 (%) Gait Score 0 (%) Gait Score 1–5 (%)
No 9.12 60.48 19.71 18.00 0.91 0.31 3.18 77.3 22.7 97.8 2.20
Yes 9.31 55.73 20.55 18.70 0.89 0.37 3.13 76.3 23.7 97.5 2.47
SEM 0.10 2.72 1.74 1.63 0.008 0.037 0.08 1.1 1.1 0.6 0.55
0 9.52 53.80 25.50 23.64 0.92 0.47 3.33 a 72.2 27.8 96.1 b 3.85 a
250 9.37 62.41 13.33 11.68 0.87 0.19 3.17 ab 75.5 24.5 98.4 a 1.60 b
500 8.74 58.09 21.56 19.74 0.90 0.35 2.96 b 82.7 17.3 98.4 a 1.56 b
SEM 0.11 3.33 2.13 1.99 0.010 0.045 0.09 1.4 1.4 0.6 0.62
No 0 9.25 bc 58.70 17.43 bc 15.83 cb 0.95 ab 0.28 b 3.33 70.2 c 29.8 c 96.3 3.71
No 250 9.27 abc 62.20 14.35 bc 12.85 cb 0.93 ab 0.21 b 3.26 77.8 abc 22.2 abc 98.7 1.34
No 500 8.85 dc 60.53 27.35 ab 25.33 ab 0.97 a 0.43 ab 2.95 83.9 a 16.1 a 98.4 1.56
Yes 0 9.80 a 48.90 33.58 a 31.45 a 0.98 a 0.67 a 3.33 74.2 bc 25.8 bc 96.0 4.00
Yes 250 9.48 ab 62.63 12.30 c 10.50 c 0.89 b 0.17 b 3.08 73.2 c 26.8 c 98.1 1.86
Yes 500 8.64 d 55.65 15.78 bc 14.15 bc 0.92 ab 0.26 b 2.97 81.5 ab 18.5 ab 98.4 1.56
SEM 0.15 4.71 3.01 2.82 0.014 0.063 0.13 1.9 1.9 0.8 0.79
Source of variation ----------------------------- Probability ---------------------
Xylanase 0.08 0.23 0.74 0.76 0.08 0.16 0.62 0.78 0.78 0.69 0.69
Glycan PB <0.001 0.22 0.002 0.002 <0.001 <0.001 0.05 <0.001 <0.001 0.03 0.03
Xylanase*Glycan PB 0.04 0.57 0.001 0.001 <0.001 <0.001 0.67 0.05 0.05 0.92 0.92

a–d Means with different superscripts differed at p < 0.05. Superscripts are only shown when main effect of treatment had a p < 0.05. 1 Produced by the catalytic oligomerization of food sugars into tailored glycans [3,4] (Midori USA, Inc., Cambridge, MA, USA, DSM Nutritional Products, Kaiseraugst, Switzerland).