KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| goat anti-GluD2 | Santa Cruz Biotechnology | Cat# sc-26118; RRID:AB_2114046 |
| goat anti-GluD2 | Frontier Institute | RRID: AB_2571602 |
| rabbit anti-HA | Cell Signaling Technology | Cat# 3724;RRID:AB_1549585 |
| guinea pig anti-vGluT1 | Millipore EMD | Cat# AB5905; RRID:AB_2301751 |
| guinea pig anti-vGluT1 | Frontier Institute | RRID: AB_2571602 |
| guinea pig anti-vGluT2 | Millipore EMD | Cat# AB2251-I; RRID:AB_2665454 |
| guinea pig anti-vGluT2 | Frontier Institute | RRID: AB_2571621 |
| chicken anti-GFP | Aves Labs | Cat# GFP-1020; RRID:AB_2307313 |
| rabbit anti-DsRed/tdTomato | Clontech/Takara Bio | Cat# 632496; RRID: AB_10013483 |
| Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) antibody | Jackson ImmunoResearch Labs | Cat# 703-545-155; RRID:AB_2340375 |
| Cy3 Donkey Anti-Rabbit IgG antibody | Jackson ImmunoResearch Labs | Cat# 711-165-152; RRID:AB_2307443 |
| Alexa Fluor 647 Donkey Anti-Goat IgG antibody | Jackson ImmunoResearch Labs | Cat# 705-605-003; RRID:AB_2340436 |
| DyLight 405 Donkey Anti-Rabbit IgG antibody | Jackson ImmunoResearch Labs | Cat# 711-475-152; RRID:AB_2340616 |
| Alexa Fluor 647 Donkey Anti-Rabbit IgG antibody | Jackson ImmunoResearch Labs | Cat# 711-605-152; RRID:AB_2492288 |
| Alexa Fluor 647 Donkey Anti-Guinea Pig IgG antibody | Jackson ImmunoResearch Labs | Cat# 706-605-148; RRID:AB_2340476 |
| Alexa Fluor 488 Donkey Anti-Guinea Pig IgG antibody | Jackson ImmunoResearch Labs | Cat# 706-545-148; RRID:AB_2340472 |
| Cy3 Donkey Anti-Guinea Pig IgG antibody | Jackson ImmunoResearch Labs | Cat# 706-165-148; RRID:AB_2340460 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Isoflurane | Henry Schein Animal Health | CAS# 26675-46-7; CHEBI:6015 |
| Avertin (2,2,2-Tribromoethanol) | Sigma | SKU# T48402 |
| DAPI | ThermoFisher Scientific | Cat# D1306 |
| Fast Green dye | Millipore Sigma | Cat# F7258 |
| Buprenorphine-SR | ZooPharm | lot # BSRLAB0.5-191112 |
| Ritodrine hydrochloride | Sigma | R0758 |
| Triton X-100 | Millipore Sigma | T8787 |
| Fluoromount-G | ThermoFisher Scientific | Cat# 00-4958-02 |
| normal donkey serum | Jackson ImmunoResearch Labs | Cat# 017-000-121; RRID:AB_2337258 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Mouse: Crl:CD1(ICR) | Charles River | RRID:IMSR_CRL:022 |
| Mouse: Ai14 | JAX | JAX stock #007908; RRID:IMSR_JAX:00 7908 |
| Mouse: GluD2-fl | Takeuchi et al., 2005 | N/A |
| Mouse: Cbln1-fl | T.C. Sudhof/J.I. Morgan | N/A |
| Mouse: Hprt-Cre | JAX | JAX stock #004302; RRID:IMSR_JAX:00 4302 |
| Mouse: Nestin-Cre8.5 | Petersen et al., 2002 | N/A |
| Mouse: MADM6-GT | Contreras et al., 2020 | N/A |
| Mouse: MADM6-TG | Contreras et al., 2020 | N/A |
| Oligonucleotides | ||
| LacZ-sg1 target sequence: TGCGAATACGCCCACGCGAT | This paper | N/A |
| LacZ-sg2 target sequence: CGGCGCGTAAAAATGCGCTC | This paper | N/A |
| Grid2-sg1 target sequence: TGATAGAATCAGCGGTCGCC | This paper | N/A |
| Grid2-sg2 target sequence: CCTTCTGTTGGTCTCGAACC | This paper | N/A |
| Recombinant DNA | ||
| pCAG-Cre | Matsuda and Cepko, 2007 | RRID:Addgene_13775 |
| pCAG-GFP | Matsuda and Cepko, 2004 | RRID:Addgene_11150 |
| pAAV-CAG-eGFP | E. Boyden, unpublished | RRID:Addgene_37825 |
| pCAG-tdTomato | W. Joo | N/A |
| pCAG-2xHA-GluD2WT | This paper | N/A |
| pCAG-2xHA-GluD2-DIER | This paper | N/A |
| pX333 | Maddalo et al., 2014 | RRID:Addgene_64073 |
| pX333-CBh-Cas9-U6-sgRNA-LacZ | This paper | N/A |
| pX333-CBh-Cas9-U6-sgRNA-GluD2 | This paper | N/A |
| Software and Algorithms | ||
| ZEN | Carl Zeiss | RRID: SCR_013672 |
| Imaris 9.3 | Oxford Instruments | RRID:SCR_007370; https://imaris.oxinst.com/ |
| ImageJ (Fiji) | NIH | https://imagej.net/Fiji/Downloads |
| Prism 8.4 | GraphPad | RRID:SCR_002798; https://www.graphpad.com/ |
| Excel | Microsoft | RRID:SCR_016137 |
| Python | Python Software Foundation | https://www.python.org |
| Other | ||