Figure 1.

RNA-Seq from dolphin peripheral blood mononuclear cells reveals enrichment of pathways similar to humans. (A) Whole blood from dolphins undergoing fasted breath holds at baseline (0–30 s), 3 min, and 4.5 min was collected from tail flukes and stored in PAXgene tubes for RNA extraction of peripheral blood mononuclear cells and RNA-Seq. (B) Gene set enrichment analysis of baseline RNA-Seq data ranked by total expression pinpoints highly expressed relevant pathways. (C) Enrichment plots for heme metabolism, coagulation and IL6/JAK/STAT3 signaling from baseline dolphin RNA-Seq data. (D) GSEA-based pathway enrichment from GTEx human whole blood RNA-Seq data ranked by total expression. (E) GSEA enrichment plots comparing dolphin RNA-Seq data ranked by total expression with top 100 and top 500 expressed genes in human whole blood.