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. 2022 Feb 2;14(3):779. doi: 10.3390/cancers14030779

Figure 4.

Figure 4

Vincristine treatment reduces tubulin acetylation in H3122, but not in H2228 cells. (A,B). H3122 and H2228 cells were treated with vincristine (20 nM) at the indicated time points before fixated and stained with anti-acetylated tubulin (green), anti-α-tubulin (red) and DAPI (blue). Scale bars, 10 μm. (C). Box plot shows the intensity of acetylated tubulin from (A,B). Data represent counts from >20 cells, n = 2. (D). H3122 and H2228 cells were treated with vincristine at the indicated time points. Western blots of acetylated tubulin are shown. GAPDH was used as a loading control. * p < 0.05, ns = not significant.