Figure 5.

The combination of ALK inhibitor and vincristine impairs downstream signalling of RAS/MAPK, PI3K/AKT and JAK/STAT3 in H3122 but not H2228 cells. (A). H3122 and H2228 cells were serum starved overnight and then treated with the indicated inhibitors for 3 h (crizotinib/ceritinib 400 nM and vincristine 20 nM). Western blotting analysis for the indicated proteins was performed. β-actin was used as a loading control. (B–E). ELISA assay was used to quantify expressions of pALK^Y1604, pSTAT3^Y705, pAKT^S473 and pERK^T202/Y204 proteins. Data represent counts from three biological replicates, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 in comparison to DMSO by one-way ANOVA. ns = not significant.