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. 2022 Feb 2;11(3):516. doi: 10.3390/cells11030516

Figure 6.

Figure 6

Plasma-derived EVs from FTLD patients are enriched in pathological TDP-35 and contain CASA complex components. Analysis of LVs and SVs from plasma of healthy controls and FTLD patients (n = 3). (A,C) Representative NTA analysis of LVs (A) and SVs (C) from plasma of a healthy control. x-axis = vesicles dimension, expressed in nm; y-axis = vesicles concentration, expressed as number of particles/mL. LV, mean size = 180.2 ± 3.9 nm; SV, mean size = 154.1 ± 1.7 nm. (B,D) Representative TEM images of LVs (one LV of approximately 200 nm) and SVs (three SVs of approximately 100–150 nm) from plasma of a healthy control (Scale bar: 500 nm). (E–K) WB and relative quantifications of LVs (F–J) and SVs (L–P) samples from plasma of three healthy controls (c1–c2–c3) and three FTLD (f1–f2–f3) patients. Samples were analyzed for TDP-43 species, Annexin I, ALIX, HSP70, BAG3 and HSPB8. Bar graphs represent the mean optical density ± SD of a specific protein normalized on the optical density of the internal housekeeping protein (annexin I for LVs and ALIX for SVs) and reported as relative to control (ctrl) group, for three biological replicates. (* p < 0.05, unpaired one-tailed t-test).