Table 7.
Summary of methodologies that used new materials in the sample preparation for the analysis of TAs.
| Analyte | Sample (Amount) | SLE Conditions | SPE Conditions | Other Treatments | Recovery (%) | [Ref.] |
|---|---|---|---|---|---|---|
| At, Sc + 2 TAs | Przewalskia tangutica fruit (1 g) | 3 mL NH3 + vortex (2 min) + 30 mL ACN + agitation (30 min) + centrifuged (5 min, 8000 rpm) × 3 Evaporated + dissolved in MeOH (1 mL) before MISPE |
MIP (100 mg) C: ACN (3 mL) L: supernatant (1 mL) W: ACN saturated n-hexane (1 mL) E: HAC/MeOH (3/7, v/v) (2 mL) |
- | 82–102 TAs 97–102 At 95–98 Sc |
[49] |
| Sc | Plants (Hindu Datura, Belladona and Hyoscyamus niger) (50 mg) |
250 mL HCl (2 M) + ultrasound (2 h) 10 mL supernatant + NH3 (pH 8–9) + dichloromethane + dried + dissolved with MeOH (50 mL) before MISPE |
MIP (100 mg) C: MeOH (10 mL) + dichloromethane (5 mL) L: supernatant (10 mL) W: MeOH/ethyl acetate (10/90, v/v) (5 mL) E: MeOH/H2O (2% HAC) (60/40, v/v) (2 mL) |
Evaporated + reconstituted in dichloromethane | 96–106 | [50] |
| At, Sc | Gluten-Free grains and flours (1 g) | 8 mL H2O (1.1% HCl, pH 1.0) + agitation (30 min) + centrifuged (10 min, 6000 rpm) + precipitate washing 1 mL H2O (1.1% HCl, pH 1.0) + filtration before SPE |
M-SBA-15-SO3− (150 mg) C: H2O (1.1% HCl, pH 1.0) (5 mL) L: supernatant (9 mL) W: H2O (1.1% HCl, pH 1.0) (3 mL) E: MeOH (3 mL) + MeOH with 10% ammonia solution (90:10, v/v, pH 11.8) (6 mL) |
Evaporated under vacuum + reconstituted in ACN/H2O (50:50, v/v) | 93–105 At 93–96 Sc |
[14] |
| At, Sc | Thyme, basil, coriander (0.25 g) |
8 mL H2O (1.1% HCl, pH 1.0) + agitation (30 min) + centrifuged (10 min, 6000 rpm) + precipitate washing 1 mL H2O (1.1% HCl, pH 1.0) + filtration before SPE |
HMS-SO3− (75 mg) C: H2O (1.1% HCl, pH 1.0) (5 mL) L: supernatant (9 mL) W: H2O (1.1% HCl, pH 1.0) (3 mL) E: MeOH (3 mL) + MeOH with 10% ammonia solution (90:10, v/v, pH 11.8) (6 mL) |
Evaporated under vacuum + reconstituted in ACN/H2O (50:50, v/v) | 87–92 At 70–92 Sc |
[28] |
Abbreviations: ACN: acetonitrile. At: Atropine. C: conditioning. E: elution. HAC: acetic acid. L: Loading. MeOH: Methanol. MIP: molecularly imprinted polymer. MISPE: molecularly imprinted solid-phase extraction. Sc: scopolamine. SLE: solid-liquid extraction. SPE: solid phase extraction. TAs: tropane alkaloids. W: washing. -: Other treatmeants have not been applied.