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. 2022 Jan 20;11(3):336. doi: 10.3390/cells11030336

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SPAG6 and SPAG6L have different binding partners. Direct yeast two-hybrid assay to analyze interaction between the two SPAG6 proteins and TAC-1 (left) and COPS5 (right). Indicated plasmids were transformed into AH109 yeast. The transformed yeast grew on the plates with non-selection medium (SD-2) and selection medium (SD-3). Pair of P53/LgT was used as the positive control. Notice that both SPAG6 proteins interacted with TAC-1 in the assay; however, COPS5 only interacted with SPAG6L. The images are representative of three independent experiments.