Figure 3.

Serine 73 phosphorylation of MITF affects MITF-PDH interaction during mast cell stimulation. (A), IP of MITF in IgE-DNP activated RBL cell lysates in the presence and absence of ERK1/2 inhibitor. Cell lysates were pre-treated with U0126 as described in Figure 2. Non-sensitized cells are presented as Control (Q: Quiescent). For IP anti-MITF antibody was used and for Western blot analysis, anti-pS73-MITF, anti-MITF, anti-PDHA antibodies were used. (B), Densitometry of PDHA/MITF levels in RBL cells after IP experiment. A two-tailed Student t-test was performed for all the western blots. Results represent means ± SEMs (n = 4, ** p ≤ 0.01; * p ≤ 0.05; ns p > 0.05).